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February 11, 2011
frustrations
I have just wasted half a day trying to do something for which there is already a web tool. So, for the next person doing PCR cloning:
To create primers:
Invitrogen's Primer Design
DNAstar PrimerSelect (paid)
To look at protein motifs:
Motif
Or, just use "Identify Conserved Domains" link on NCBI
To test your primers for possible other products:
PrimerBlast
Or, better yet, get your advisor to pay for a new version of DNAstar instead trying to use workarounds around old software.
Also, stupid things you might do: forget to check the reading frame. However, if you are using PrimerSelect, the program automatically selects based on the first reading frame.
Posted by azileretsis at February 11, 2011 04:48 PM
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